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PrimeSpot test can accurately diagnose infections from different viruses, Lyme disease and chronic fatigue syndrome using sensitive molecular biology techniques (quantitative Polymerase Chain Reaction – qPCR). During the PrimeSpot test, a patient’s DNA and RNA is isolated from blood cells and serum. We then use them as a template in both one-step and two-step RNA qPCR reactions. The PrimeSpot test is highly sensitive and can detect and quantify certain viruses, and the presence of Lyme disease-associated species.